HUBUNGAN PEMBENTUKAN BIOFILM OLEH BAKTERI GRAM NEGATIF DENGAN RESISTENSI ANTIBIOTIK PADA WANITA DIABETES MELITUS TIPE 2
Abstract
Diabetes melitus (DM) memiliki efek jangka panjang terhadap sistem genitourinari yang menjadi faktor predisposisi terjadinya infeksi saluran kemih (ISK) pada pasien wanita dan umumnya bersifat asimtomatik. Bakteri gram negatif merupakan mikroorganisme penyebab tersering yang dapat membentuk biofilm sehingga sering menyebabkan resistensi antibiotik. Penelitian ini bertujuan untuk membuktikan hubungan antara pembentukan biofilm bakteri gram negatif dengan resistensi antibiotik pada wanita diabetes melitus tipe 2 (DMT2). Menggunakan desain penelitian cross sectional dengan teknik total sampling. Jumlah sampel penelitian adalah 45 orang wanita DMT2 anggota Program Pengendalian Penyakit Kronis (PROLANIS). Pada semua responden dilakukan kultur urin porsi tengah, uji sensitivitas dan pemeriksaan biofilm terhadap isolat bakteri yang teridentifikasi. Hasil kultur urin menunjukkan bakteriuria signifikan 14 (31%) responden. Identifikasi koloni menunjukkan bakteri penyebab ISK antara lain Escherichia coli (35,7%), Klebsiella pneumoniae (35,7%), Enterobacter sp (21,5%) dan Citrobacter sp (7,1%). Uji sensitivitas dijumpai 8 (57%) isolat resisten terhadap antibiotik, yaitu 2 (14,2%) isolat terhadap ciprofloksasin dan 6 (42,8%) isolat terhadap TMP-SMX. Bakteri gram negatif yang diisolasi 100% mampu membentuk biofilm dengan kategori weak. Berdasarkan analisis bivariat dengan uji Rank Spearman correlation dapat disimpulkan bahwa terdapatnya hubungan yang sangat lemah antara pembentukan biofilm oleh bakteri gram negatif dengan resistensi antibiotik ciprofloksasin dan TMP-SMX.
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Rachman, N. O., Muhammad Darwin, P., & Lia Yuli, B. (2016). Bacterial Sensitivity Test Causes Urinary Tract Infection in Diabetes Mellitus Patients Against: Ceftriaxone, Levofloxacin and Gentamicin. Medical periodical journal, 205-213.
World Health Organization. (2016). Diabetes Facts and Numbers. World Helath Diabetes Days.
Samuel, O. O., Mathew, A. O., Agboola, D., Joshua, O., Tosin, A., Grace, A. B., et al. (2014). Asymptomatic Urinary Tract Infection In Diabetic Patients In Ago –Iwoye, Ogun State, Nigeria. Journal of American Science, 72-78.
Ariwijaya, M., & Suwitra, K. (2007). Prevalence, Characteristics and Factors Associated with Urinary Tract Infection in Inpatient Diabetes Mellitus Patients. Journal of Internal Medicine, 112-127.
Schneeberger, C., Kazeimer, B. M., & Suzzane, G. A. (2014). Asymptomatic Bacteriuria And Urinary Tract Infections In Special Patient Groups: Women With Diabetes Melitus And Pregnant Women. Curr Opin Infect Dis, 27, 108-114.
Flores-Mireles, A. L., Walker, J. N., Caparon, M., & Caparon, M. (2015). Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat Rev Microbiol, 269-284.
Nitzan, O., Elias, M., & Saliba, B. C. (2015). Urinary Tract Infection in Patient with Type 2 Diabetes Melitus: Review of Prevalence, diagnosis and Management. Dovepress Journal, 129-136.
Hernandez, J. G. (2013). Doctoral Dissertation :Mechanism of Microbial - Host Interaction During Asymptomatic Bacteriuria. Sweden: Lund University.
Saptiningsih, M. (2012). Determinan Urinary Tract Infection Patient Diabetes Mellitus Women in RSB Bandung. Thesis. Master of Nursing UI.
Srinivas, A., Chandrashekar, U. K., Shivaskhankara, K. N., & Pruthvi, B. C. (2014). Clinical Profile of Urinary Tract Infections in Diabetic and Non-Diabetics. Australasian Medical Journal (AM), 29-34.
Divyashree, K., & Yadhav., K. (2015). Asymptomatic Bacteriuria and Associated Host Factors in Diabetic Patients with Special Reference to UTI Chromagar. International Journal of Medical Research and Review, 1218-1223.
Boyko, E. J., Fihn, S. D., Scholes, D., Abraham, L., & Monsey, B. (2005). Risk of Urinary Tract Infection and Asimtomatic Bacteriuria among Diabetic and Nondiabetic Postmenauposal Women. Am J Epidemiol, 557-564.
Al-Rubeaan, K. A., Moharram, O., Al-Naqeb, D., & RaWullah, A. H. (2013). Prevalence of Urinary Tract infection and Risk Factors among Saudi Patients with Diabetes. World J Urol , 573–578.
Oliveira, M. C., Varella, L. R., Angelo, P. H., & Micussi, M. T. (2016). The Relationship between The Presence of Lower Urinary Tract Symptoms and Waist Circumference. Diabetes Metab Syndr Obes., 207–211.
Renko, M., Tapanainen, P., Tossavainen, P., Tytti, P., & Matti, U. (2011). Review/ Commentaries ADA Statement: Meta-Analysis of the Significance of Asymptomatic Bacteriuria in Diabetes. Diabetes Care, 34(1).
Reygaert, W. C. (2014). Innate Immune Response to Urinary Tract Infections Involving Escherichia coli. Journal of Clinical and Cellular Immunology, 1-5.
Genghesh, K. S., Elkateb, E., Berbash, N., Nada, R. A., Ahmed, S. F., Rahouma, A., et al. (2009). Uropathogen from Diabetic Patients in Libya: Virulence factors and Phylogenetic groups of Escherichia coli Isolates. Journal of Medical Microbiology, 1006-10014.
Sobeiszczyk, M. E. (2009). Urinary Tract Infection. Dipetik September 17, 2017, dari columbia.edu: http://www.columbia.edu/itc/hs/medical/pathophys/id/2009/utiColor.pdf
Rahmad, B. (2017). Thesis: Isolation and Identification of Ciprofloxacin Resistance Genes in Isolate Escherichia coli Multidrug Resistance of Persons with Urinary Tract Infection in Abdoell Moeloek Hospital Lampung Province. Post Graduate Program Master of Chemistry Faculty of Mathematics and Natural Sciences.
Nigussie, D., & Amsalu, A. (2017). Prevalence of Uropathogen and Their Antibiotic Resistance Pattern among Diabetic Patients. Turkish Journal of Urology, 85-92.
Sayal, P., Devi, P., & Singh, K. (2016). Bacterial Colonization and Biofilm Formation among Diabetic Patients: A Therapeutic Challenge Int.J.Curr.Microbiol.App.Sci , 174-181 .
Fattahi, S., Kafil, H. S., Nahai, M. R., Asgharzadeh, M., Nori, R., & Aghazadeh, M. (2015). Relationship of Biofilm Formation and Different Virulence Genes in Uropathogenic E. coli Isolated from Northwest Iran. GMS Hyg Control, 1-7.
Wood, T. K. (2009). Insight on E.coli Biofilm Formation and Inhibition from Whole Transcriptome Profiling . HHS Public Access Environ Microbiol, 1-15.
Priyadharshini, A., Mangaiyarkarasi, Balasubramaniam, Pragash, D. S., & Gopal. (2014). Biofilm Production and Antibiotic Resistance among Uropathogens Causing Bacteriuria in Diabetic Individuals. Scholars Journal of Applied Medical Science (SJAMS), 568-571.
Alves, M. J., Barreira, J. C., Carvalho, I., Trinta, L., Pereira, L., Fereira, I. C., et al. (2014). Prospensity for Biofilm Formation by Clinical Isolates from UTI: Developing a Multifactorial Predictive Model to Improve Antibiotheraphy. Journal of Medical Microbiology, 471-477.
Yenny, & Herwana, E. (2007). Resistance ofEnteric Bacteria: Global Aspects of Antimicrobials. Universa Medicina, 46-56.
DOI: https://doi.org/10.29103/averrous.v4i1.804
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