EFEK PEMBERIAN KALSITRIOL (1α,25 (OH)2 D3) TERHADAP KADAR CYCLIC AMP KULTUR KARDIOMIOSIT

Authors

  • Cut Sidrah Nadira Prodi Pendidikan Dokter Fakultas Kedokteran Univeristas Malikussaleh

DOI:

https://doi.org/10.29103/averrous.v1i2.397

Keywords:

kalsitriol, kontraktilitas, kardiomiosit, troponin I

Abstract

Defisiensi kalsitriol dapat meningkatkan faktor risiko terjadinya penyakit kardiovaskular. Hal ini dikaitkan dengan penurunan fosforilasi troponin I (TnI) di Ser23/24. Fosforilasi TnI di Ser23/24 berperan dalam regulasi Mg-ATPase miofilamen dan sensitivitas miofilamen terhadap Ca2+ yang selanjutnya mempengaruhi respon frekuensi dan kekuatan ejeksi ventrikel. Ser23/24 TnI ini diketahui merupakan substrat spesifik PKA. Penelitian ini bertujuan untuk mempelajari apakah efek kalsitriol terhadap peningkatan fosforilasi TnI mempunyai jalur mekanisme aksi yang juga melalui cAMP/PKA. Penelitian ini merupakan eksperimental murni menggunakan rancangan pre-post test with control group dengan sampel kultur primer kardiomiosit Sprague-Dawley jantan dewasa. Kardiomiosit diinkubasi kalsitriol selama 5 menit kemudian kadar cAMP diukur menggunakan metode ELISA. Hasil penelitian tidak menunjukkan adanya korelasi antara peningkatan TnI terfosforilasi dengan kadar cAMP (p>0,05). Penelitian ini masih belum dapat membuktikan adanya jalur aktivasi cAMP/PKA oleh kalsitriol dalam mekanisme peningkatan TnI terfosforilasi pada kardiomiosit.

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Published

2018-02-16

Issue

Averrous, Vol. 1: No. 2 (November, 2015)

Section

Articles

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