The Antiacne Activity of Sunkist Peels Methanol Extract in Propionibacterium Acne-Induced Acne Vulgaris

Maya Sari Mutia, Etha Wilda Hasiani Damayanti, Tan Suyono, Widya Yanti Sihotang


Treatment with acne vulgaris is a challenge because the complex pathophysiology of acne vulgaris. One of the natural products that potentially use as a herbal medicine is Sunkist peel. The peel of Sunkist is more inclined to end up as waste from domestic industries. The aim of this study was to investigate the antiacne activity of the Sunkist peel methanol extract gel in vivo study. The experimental study used by applied Sunkist peel extract gel to rats injected intradermally with Propionibacterium acne suspension. One hour before the injection of Propionibacterium acnes suspension, all rats were applied to a topical gel based on the group. Control, standard, Sunkist Peel Extract Gel-I, II, and III groups received gel base, clindamycin, 5%, 10%, and 15% of Sunkist peel extract gel, respectively. This study indicates the methanol extract of Sunkist orange peel with a yield of 8.73% revealed the presence of some phytochemicals, including alkaloids, flavonoids, tannins, and saponins. Sunkist orange peel methanol extract gel significantly reduced the size of acne vulgaris lesions after 7-14 days of extract application (p-value <0.05). It is in line with the histological study that the gel concentration increased, followed by the growth of the epidermal layer. This study sstrongly indicates that Sunkist orange peel methanol extract has an antiacne effect in a physically stable gel form.


skin disorders; cosmetics; vitamin a: clindamycin; mouse

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Zhou B, Carrillo-Larco RM, Danaei G, et al. Worldwide trends in hypertension prevalence and progress in treatment and control from 1990 to 2019: a pooled analysis of 1201 population-representative studies with 104 million participants. Lancet. 2021;398:957-980. doi:10.1016/S0140-6736(21)01330-1

Khairunnisa K, Rialita A, Mardhia M. Pengetahuan dan Perilaku Kebersihan Wajah Terhadap Timbulnya Akne Vulgaris pada Pelajar SMP di Mempawah Hilir. J Kedokt dan Kesehat Publ Ilm Fak Kedokt Univ Sriwij. 2021;8(1):25-32. doi:10.32539/v8i1.11886

Agarwal S, Pillai A, Singh AP, Pareek R, Bhuptani N V. Drug Utilization Study of Medications Used for Acne Vulgaris in a Tertiary Care Hospital. Eur J Biomed Pharm Sci. 2016;3(11):311-315.

Al-Mekhlafi R, Attiyah RE, Haddad YR, Salah LA. Awareness on Teratogenic Effects of Isotretinoin and Compliance with Precautionary Measures among Women of Childbearing Age in Makkah Province, Saudi Arabia. Obstet Gynecol Int. 2021;2021:1-6. doi:10.1155/2021/9966300

Castellanos Lorduy HJ, Pérez Cely HC, Casadiego Rincón EJ, Henao Riveros SC, Colorado CL. Cutibacterium acnes Tetracycline Resistance Profile in Patients with Acne Vulgaris, in a Colombian Dermatologic Center. Actas Dermosifiliogr. 2021;112(10):873-880. doi:10.1016/j.adengl.2021.09.003

Pandey M, Qidwai A, Pandey A, et al. Investigation for In Vitro Antibacterial Activity of Citrus sinensis L. against Propionibacterium acnes. Int J Curr Adv Res. 2017;6(8):5400-5404.

Michiko M, Manalu CV, Mutia MS. Uji Efektivitas Ekstrak Etanol Kulit Jeruk Manis (Citrus sinensis) terhadap Bakteri Propionobacteriu acnes. (Jurnal Ilm Mhs Kesehat Masyarakat). 2020;5(1). doi:10.37887/jimkesmas.v5i1.10552

Ariani LW, Wigati D. Formulasi Masker Gel Peel-Off Ektrak Etanol Kulit Buah Jeruk Manis (Citrus sinensis (L.) Osbeck) Sebagai Obat Jerawat. Media Farm Indones. 2016;11(2):1084-1092.

Mutia MS, Manalu CV. Uji Efektivitas Ekstrak Etanol Kulit Jeruk Manis (Citrus Sinensis) Terhadap Bakteri Vibrio cholerae. J Prima Med Sains. 2020;2(1):6-9. doi:10.37887/jimkesmas.v5i1.10552

Mutia MS, Suhartina. Role of Sunkist Peels Extract Against the Level of Estradiol and Maturity of Follicle in the Female Wistar Rat. Asian J Pharm Clin Res. 2021;14(4):120-123. doi:10.22159/ajpcr.2021.v14i4.40788

Gulo KN, Suhartomi, Saragih AD, Raif MA, Ikhtiari R. Antioxidant Activity of Flavonoid Compounds in Ethanol and Ethyl Acetate Extract from Citrus Sinensis. In: 2021 International Conference on Artificial Intelligence and Mechatronics Systems (AIMS). IEEE; 2021:1-6. doi:10.1109/AIMS52415.2021.9466078

Chiuman L, Ginting CN, Yulizal OK. Lemon Pepper Extract Improves Diabetic Nephropathy in Diabetic Rats. Open Access Maced J Med Sci. 2022;10(A):170-175. doi:10.3889/oamjms.2022.8226

Mawarni E, Ginting CN, Chiuman L, Girsang E, Handayani RAS, Widowati W. Antioxidant and Elastase Inhibitor Potential of Petals and Receptacle of Rose Flower (Rosa damascena). Pharm Sci Res. 2020;7(2):105-113. doi:10.7454/psr.v7i2.1016

Widowati W, Wargasetia TL, Afifah E, et al. Antioxidant and antidiabetic potential of Curcuma longa and its compounds. Asian J Agric Biol. Published online 2018.

Aslani A, Zolfaghari B, Davoodvandi F. Design, formulation and evaluation of an oral gel from Punica granatum flower extract for the treatment of recurrent aphthous stomatitis. Adv Pharm Bull. 2016;6(3):391-398. doi:10.15171/apb.2016.051

Sing R, Bansal S, Mishra MK. Formulation and Evaluation of Herbal Oral Gel Containing Extracts of Powdered Psidium guajava Linn Leaves with Curcuma longa Linn Rhizomes to Treat Mouth Ulcer. Int J Drug Dev Res. 2020;12(2):1-7. doi:10.36648/0975-9344.12.2.150

Chen LW, Chung HL, Wang CC, Su JH, Chen YJ, Lee CJ. Anti-acne effects of cembrene diterpenoids from the cultured soft coral sinularia flexibilis. Mar Drugs. 2020;18(10):1-12. doi:10.3390/md18100487

Tsai TH, Huang WC, Lien TJ, et al. Clove extract and eugenol suppress inflammatory responses elicited by Propionibacterium acnes in vitro and in vivo. Food Agric Immunol. 2017;28(5):916-931. doi:10.1080/09540105.2017.1320357

Agarwal P, Tyagi N, Gaur PK, Puri D, Shanmugam SK. Polyherbal anti acne gel containing extracts of Mangifera indica and Syzygium cumini seeds: Bioassay guided activity against Propionibacterium acne. Biointerface Res Appl Chem. 2019;9(4):4177-4182. doi:10.33263/BRIAC94.177182

Pandey A, Tripathi S. Concept of Standarization, Extraction and Pre Phytochemical Screening Strategies for Herbarl Drug. J Pharmacogn Phytochem. Published online 2014.

Depari SAF, Rambe DJA, Meilando R, Lisya C, Mutia MS, Lubis YEP. Uji Efektivitas Ekstrak Etanol Kulit Jeruk Sunkist (Citrus Sinensis (L.) Osbeck) Terhadap Kadar Gula Darah Tikus Wistar (Rattus Norvegicus) Dengan HiperkolesterolemiaI yang Diinduksi Streptozotocin. Biospecies. 2021;14(1):1-9.

Nawangsari D, Sunarti. Uji Stabilitas Sediaan Salep Ekstrak Etanol Rimpang Kencur. J Pharmacopolium. 2021;4(2):67-74.

Wahdaningsih S, Untari EK. The Antibacterial Activity of Red Dragon Fruit Peel (Hylocereus polyrhizus Britton & Rose) Methanolic Fraction Against Staphylococcus epidermidis and Propionibacterium acnes. J Pharmascience. 2021;8(2):51-58. doi:10.20527/jps.v8i2.10378

Eldahshan OA, Abdel-Daim MM. Phytochemical study , cytotoxic , analgesic , antipyretic and anti-inflammatory activities of Strychnos nux - vomica. Cytotechnology. 2015;67:831-844. doi:10.1007/s10616-014-9723-2

Gaichu DM, Mawia AM, Gitonga GM, Ngugi MP, Mburu DN. Phytochemical screening and antipyretic activities of dichloromethane-methanolic leaf and stem bark extracts of Ximenia americana in rat models. J Herbmed Pharmacol. 2017;6(3):107-113.


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