Pengaruh Letak Posisi Eksplan dan Sitokinin Pada Perkecambahan Biji Manggis (Garcinia mangostana L.) Lokal Aceh Secara in-Vitro
DOI:
https://doi.org/10.29103/agrium.v14i2.874Abstract
The application of modern biotechnology is needed in order to enhance the genetic diversity of mangosteens. The application of tissue culture on mangosteens is an important thing to do to support the biotechnology program of mangosteen plants. The purpose of this research is to reveal the influence of location of explants and Benzylaminopurine (BAP) concentration on germination of local Acehnese mangosteens in vitro. This research was conducted at the Laboratory of tissue culture of the Faculty of Agriculture, Malikussaleh University from March to May 2017. The study used an environmental design in the form of Completely Randomized Design (CRD) with two factors. The first factor is the location of explant position is abaxial (L1) and adaxial (L2). The second factor was the concentration of BAP 0 mg / L (B0), 2.5 mg / L (b1), and 5 mg / L (B2). The results showed that the position of eksplan gave an effect on mangosteen germination in vitro for its shoot length variables. The explant positions that had the best effect on the growth of in vitro mangosteen sprouts were adaxial. BAP concentration had an effect on germination of mangosteen in vitro for its bud growth and life percentage. 2.5 ppm of BAP concentration was the best concentration for mangosteen seed growth in vitro. There was no interaction between eksplan position and BAP concentration in all observed variablesReferences
Ashari dan Sunarsih. 2006. Manggis Komoditas Unggulan Tasikmalaya. Warta Penelitian dan Pengembangan Pertanian. 28 (1) : 27-28.
[BPS} Badan Pusat Statistik. 2013. Statistik Indonesia. Badan Pusat Statistik, Aceh Utara.
George, E.F and Sherington, P.D. 1984. Plant Propogation by Tissue Culture Exegetics Limited. England.
Handayani, R.S. 2012. Rekayasa teknologi sambung mikro dan setek mikro pada tanaman manggis (Garcinia mangostana). [disertasi]. Bogor: Institut Pertanian Bogor, Program Pascasarjana. 127 hal.
Handayani, R.S., Poerwanto, R., Sobir, Purwito, A., Ermayanti, TM. 2013. Pengaruh Batang Bawah dan Jenis Tunas Pada Mikrograftting Manggis (Garcinia mangostana L.) Secara In Vitro. J. Agron. Indonesia. 41 (1) : 47-53.
Harahap, F., Poerwanto, P., Suharsono, Suriani, C., & Rahayu, S. (2014). In vitro growth and rooting of mangosteen (Garcinia mangostana l.) on medium with different concentration of plant growth regulator. HAYATI Journal of Biociences, 21 (4), 151-158.
Hartmann HT, Kester DE, Davies FT, Geneve RL. 2002. Plant Propagation Principal and Practise. Pearson Education Inc.New Jersey: Upper Saddle River.
Ihsan, F. dan Sukarmin. 2011. Teknik pengujian pembelahan biji terhadap efektifitas perbanyakan manggis (Garcinia mangostana L.) melalui biji.
Isda, M. N. 2009. Induksi Kalus Centella asiatica Melalui Aplikasi Auksin dan Sitokinin. Jerami 2(3) : 162-165.
Isharyati, 1999. Pengaruh Kadar NAA dan BAP terhadap Pertumbuhan Eksplan Ginseng Jawa (Talinum paniculatum) secara in vitro. Skripsi S1. Fakultas Pertanian Universitas Muhammadiyah. Yogyakarta.
Qosim, W. A. 2004. Pemuliaan Manggis tak Sesulit Dibayangkan. Diakses dari www.pikiran-rakyat.com/cetak/1204/cakrawala/lainnya4.htm. Tanggal 15 Desember 2016.
Qosim, W.A., Yuwariah, Y., Hamdani, J.S., Rachmadi, M. 2015. Pengaruh Mutagen Etil Metan Sufonat Terhadap Regenerasi Tunas Pasa Dua Genotip Manggis Asal Purwakarta dan Pandeglang. J. Hort. 25 (1) : 9-14.
Razdan, M.K. 2003. Introduction to Plant Tissue Culture. 2nd Edition. Science Publisher, Inc. 377 hal.
Romeida A. 2007. Respon berbagai tipe eksplan biji manggis (Garcinia mangostana L.) pada beberapa konsentrasi benzyl amino purin (BAP) terhadap pembentukan dan regenerasi polyembrioninya secara in vitro. Jurnal Akta Agrosia. 10 (2): 162-166.
Supriati, Y., N. Sunarlim, W.H. Adil, I. Mariska, Y. Rusyadi, D. Sukmadjaja, E.G. Lestari, dan Murtado. 2001. Studi regenerasi tanaman potensial dan bernilai ekonomi tinggi. Laporan Hasil Penelitian Balitbio, Bogor.
Trisnahati, A. 2007. Pengaruh Jenis Media dan Konsentrasi BAP terhadap Multiplikasi Tanaman Buah Naga (Hylocereus polyrhizus) secara in vitro. Skripsi S1. Fakultas Pertanian Universitas Sebelas Maret. Surakarta.
Widiastuti, A.,Sobir.,Suhartanto, M.R. 2013. Analisis Keragaman Genetik Manggis (Garcinia mangostana L.) Diiradiasi Dengan Sinar Gamma Berdasarkan Penanda ISSR. J. Bioteknologi Vol 10 (1): 15-22.
Yusnita. 2003. Kultur Jaringan: Cara Memperbanyak Tanaman Secara Efisien. Agromedia Pustaka: Jakarta. 105 Hal.
Downloads
Published
How to Cite
Issue
Section
License
Authors retain copyright and grant the journal right of first publication and this work is licensed under a Creative Commons Attribution 4.0 that allows others to share the work with an acknowledgement of the works authorship and initial publication in this journal.
