UJI AKTIVITAS ANTIBAKTERI DAN KARAKTERISASI METABOLIT SEKUNDER FRAKSI ETIL ASETAT SPONS Haliclona sp. ASAL PULAU LEMUKUTAN

Luthfi Imam Sulistyo; Ajuk Sapar; Rudiyansyah Rudiyansyah; Ari Widiyantoro; Puji Ardiningsih; Millenia Millenia

doi:10.29103/jtku.v13i1.15596

UJI AKTIVITAS ANTIBAKTERI DAN KARAKTERISASI METABOLIT SEKUNDER FRAKSI ETIL ASETAT SPONS Haliclona sp. ASAL PULAU LEMUKUTAN

Luthfi Imam Sulistyo, Ajuk Sapar, Rudiyansyah Rudiyansyah, Ari Widiyantoro, Puji Ardiningsih, Millenia Millenia

Abstract

Spons Haliclona sp. termasuk ke dalam kelas Demospongiae yang memiliki kandungan metabolit sekunder berupa alkaloid, terpenoid, fenolik, dan steroid. Metabolit sekunder tersebut dapat berperan sebagai antibakteri, antikanker, antiinflamasi, dan antivirus. Tujuan penelitian ini adalah mengarakterisasi metabolit sekunder dan menguji aktivitas antibakteri fraksi etil asetat terhadap Staohylococcus aureus menggunakan metode difusi sumuran. Hasil interpretasi FTIR pada isolat menunjukkan adanya serapan gugus O-H melebar (3448,72 cm^-1), =C-H (3016,67 cm^-1), C-H alifatik (2927,94 cm^-1), C-O alkohol (1118,71 cm^-1); C=C olefin (1631,78 cm^-1) C-X halogen (617,22- 410,84). Kehadiran gugus-gugus fungsi tersebut dan hasil uji fitokimia dapat diduga bahwa isolat merupakan senyawa olefin dari kelompok terpenoid. Hasil uji aktivitas antibakteri menunjukkan bahwa fraksi etil asetat menghambat bakteri Staphylococcus aureus pada konsentrasi 50 µg/µL dan 25 µg/µL masing-masing menunjukkan kapasitas kuat dalam menghambat bakteri, sedangkan pada konsentrasi 12,5 µg/µL terindikasi lemah; dan 6,25 µg/µL tidak menunjukkan aktivitas dalam menghambat bakteri S. aureus. Hasil uji fitokimia fraksi etil asetat memberikan respon positif untuk senyawa terpenoid dan alkaloid.

Keywords

Spons; Haliclona; Antibakteri; Staphylococcus aureus

Full Text:

PDF

References

Amraoui Belakkssem E., Jean François B., Fatima Ez-Zohra I., Majida El W., Mostafa Kandil, Mohammed El Amraoui, dan Aziz Fassouane, 2015, Activity of Haliscosamine Against Fusarium oxysporum f.sp. melonis: In Vitro and In Vivo Analysis, Springer Open Journal, 4(16):1-5, DOI 10.1186/s40064-4-015-0797-x.

Bhusal R. D., Nahar D. M., Dalvi P. B., 2017, Review on: Falsh Colum Chromatography, Indo American Journal of Pharmaceutical Research, 7(1): 7373-7358, ISSN 2231-6876.

Coates J., 2000, Encyclopedia of Analitycal Chemistry- Interpretation of Infrared Spectra, A Practical Approach, John Wiley & Sons Ltd, Chicester.

Damiyanti Kristina, Widiyantoro Ari, Rudiyansyah, 2020, A Prentylated Flaavonoid from the Methanol Fraction of Breadfruit Root (Artocarpus communis), Jurnal Akademia Kimia, 9(4): 237-244, E-ISSN 2477-5158.

Didimus T. B., 2015, Bakteriologi-Konsep-Konsep Dasar, Cetakan Pertama, UMM Press, Malang, ISBN: 978-979-796-329-3.

Gupta Poonam, 2019, Chemical Constituents of Haliclona: An Overview, Journal of Pharmacognosy and Phytochemistry, 8(1): 823-827, E-ISSN 2278-4136

Handayani S., Widowati R., Al Fikri A. R., 2021, Phytochemical Screening and Antibacterial Activities of Senggani

(Melastoma malabathricum L.) Ethanolic Extract Leaves, Jurnal Ilmu Pertanian Indonesia, 26(4): 562-568, e-ISSN 2443-3462.

Kalschmidt B. P., Ennen I., Greiner J. F. W., Dietsch R., Patel A., Kalschmidt C., Hütten A., 2020, Preparation of Terpenoid-Invasomes with Selective Activity against S. aureus and Characterization by Cryo Transmission Electron Microscopy, Biomedicines, 8: 105, 1-18.

Kondeti Ranjith R., Mulpuri Kranti S., Meruga Bharathi, 2014, Advancements In Column Chromatography: A Review, World Journal of Pharmacetical Sciences, 2(9): 1375-1383, E-ISSN 2321-3086.

Kumar V., 2022, Spectroscopy-1, Department of Chemistry Uttarakhand Open UniversityHaldwani, Nainital.

Kurniawan Muhammad R., Sapar Ajuk, Aritonang Anthoni B., 2021, Uji Toksisitas dan Uji Fitokimia Spons Haliclona sp. Asal Pulau Lemukutan Kabupaten Bengkayang Kalimantan Barat, Jurnal Kimia Khatulistiwa, 9(10): 1-5, ISSN 2303-1077.

Kurniawati, S., Ardiningsih, P., Widiyantoro, A., 2017, Aktivitas Antibakteri Ekstrak Daun Akar Bambak (Ipomoea sp.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli, Jurnal Kimia Khatulistiwa, 6(2): 44-50.

Latifah L. A., Tahir A., Soekamto N. H., 2021, Antibacterial Assay of Crude Extracts from Marine Sponge

Haliclona fascigera in Badi Island of Spermonde Archipelago Against Shrimp Pathogenic Bacteria, IOP Conf. Series: Earth and Environmental Science 763

Liang J., She J., Fu J., Wang J., Ye Y., Yang B., Liu Y., Zhou X., Tao H., 2023, Advances in Natural Products from the Marine-Sponge-Associated Microorganisms with Antimicrobial Activity in the Last Decade, Mar. Drugs, 21, 236.

Malanovic N and Lohner K., 2015, Gram-positive bacterial cell envelopes: The impact on the activity of antimicrobial peptides, Biochimica et Biophysica.

Millenia, Sapar A., Aritonang A. B., Rudiyansyah, and Rahmalia W., 2022, Characterization of Secondary Metabolites and Cytotoxic Assay of Haliclona sp. Sponge Against T47D Breast Cancer Cells, al Kimiya: Jurnal Ilmu Kimia dan Terapan, 10(1): 40-49, e-ISSN: 2407-1927

Murray R. K., Bender D. A., Botham K. M., Kennely P. J., Rodwell V. W., Weil P. Anthony, 2009, Harper’s Illustrated Biochemistry, 28th Edition, The McGraw-Hill Companies, United States, ISBN 978-0-07-170197-6.

Nogueira J. O., Campolina G. A., Batista L. R., Alves E., Caetano A. R. S., Brandao R. M., Nelson D. L., Cardoso M. dG., 2021, Mechanism of Action of Various Terpenes and Phenylpropanoids Against Escherichia coli and Staphylococcus aureus, FEMS Microbiology Letters, 268

Patil A. D., Kokke W. C., Cochran S., Francis T. A., Tomszek T., Westley J. W., 1992, Brominated Polyacetylenic Acids from The Marine Sponges Xestospongia muta: Inhibitor of HIV Protease, Journal of Natural Products, 55(9): 1170-1177.

Pavia D. L., Lampman G. M., Kriz G. S., 2001, Introduction to Spectroscopy – A Guide for Student of Organic Chemistry, 3th Edition, Thompson Learning, United States of America, ISBN 0-03-031961-7.

Pelu A. D. dan Djarami J., 2022, Aktivitas Antibakteri Ekstrak Etanol Daun Harendong Bulu (Clidemia hirta) asal

Maluku terhadap Staphylococcus aureus, JUMANTIKA, 7(4): 351-357.

Putra Masteria Yunovilsa dan Hadi Tri Aryono, 2017, Chemical Composition, Antimicrobial, Cytotoxic, and Antiplasmodial Activities of Three Sponges from Buton Islands, Indonesia, Ilmu Kelautan, 22(3): 147-154, ISSN 0853-7291.

Rabel Fred and Sherma Joseph, 2019, Review of Thin Layer Chromatography in Pesticide Analysis: 2016-2018, Journal of Liquid Chromatography and Related Technologies, 1-14, https://doi.org./10.1080/10826076.2018.1557055

Roge A. B., Firke S. N., Kawade R. M., Sarje S. K., Vadvalkar S. M., 2011, Brief Review on: Flash Chromatography, International Journal of Pharmaceutical Sciences and Research, 2(8): 1930-1937, ISSN 0975-8232.

Schories Dirk dan Kohlberg Gesche, 2016, Marine Wildlife King George Island Antartica, Edisi-1, Dirk Schories Publication, Rostock-Jerman, ISSN 978-00-050832-5

Sipkema Detmer, Maurice C.R. Fransen, Ronald Osinga, Johannes Tramper, Rene´ H. Wijffels, 2005, Marine Sponges as Pharmacy, Marine Biotechnology, 7: 142-162, DOI: 10.1007/s10126-004-0405-5

Shaikh Junaid R., Patil M.K., 2020, Qualitative Tests for Preeliminary Phytochemical Screening: An Overview, International Journal of Chemical Studies, 8(2): 603-608, E-ISSN 2321-1902. https://doi.org/10.22271/chemi.2020.v8.i2i.8834

Shushizadeh M. R., Behroozi S., Behfar AA. Nazemi M., 2018, Antibacterial Activity and GC-Mass Analysis of Organic Extract from Persian Gulf Haliclona sp., Pharmacophore, 9(2): 19-24.

Supriadin Asep, Kudus Rohana, dan Amalia Vina Amalia, 2017, Efek Larvasida Hasil Fraksinasi Metanol Daun Aglaia glabrata Terhadap Larva Aedes aegypti, Jurnal UIN Sunan Gunung Djati, X (1): 68-81, ISSN 1979-8911.

Tampongangoy D., Maarisit W., Ginting A. R., Tumbel S., Tulandi S., 2019, Uji Aktivitas Antibakteri Ekstrak Daun Kayu Kapur Melanolepis multiglandulosa Terhadap Bakteri Staphylococcus aureus dan Bakteri Escherechia coli, Jurnal Biofarmasetikal Tropis, 2(1): 107-114, e-ISSN 2685-3167.

Umarudin dan Yuliarni F. F., 2019, Uji Antimikroba Daging Buah (Carica pubescens) Matang Terhadap Bakteri Staphylococcus aureus Metode Kirby Bauer Secarra In Vitro, Simbiosa, 8(2): 148-157, e-ISSN 2598-6007

Wati M., Erwin, T. D., 2017, Isolasi dan Identifikasi Senyawa Metabolit Sekunder dari Fraksi Etil Asetat pada Daun Berwarna Merah Pucuk Merah (Syzgium Myrtifilium Walp.), Jurnal Kimia Mulawarman, 14(2): 100-107, E-ISSN 2476-9258.

Yadav L.D.S., 2005, Organic Spectroscopy, 1st Edition, Springer-Scince+Business media B.V., India, ISBN 978-94-2508-8.

Yan Y., Li X., Zhang C., Lv L., Gao B., Li M., 2021, Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review, Antibiotics, 10, 318.

Yasmin Y., Fitri L., Fauziah, Wasliyah F., 2019, Isolation of Bacteria from Apis cerana Hive, Their Antibacterial Potency and Cytotoxicity, Biodiversias, 20(9): 2733-2738, e-ISSN: 2085-4722

DOI: https://doi.org/10.29103/jtku.v13i1.15596