UJI AKTIVITAS ANTIBAKTERI DAN KARAKTERISASI METABOLIT SEKUNDER FRAKSI ETIL ASETAT SPONS Haliclona sp. ASAL PULAU LEMUKUTAN

Luthfi Imam Sulistyo, Ajuk Sapar, Rudiyansyah Rudiyansyah, Ari Widiyantoro, Puji Ardiningsih, Millenia Millenia

Abstract


Spons Haliclona sp. termasuk ke dalam kelas Demospongiae yang memiliki kandungan metabolit sekunder berupa alkaloid, terpenoid, fenolik, dan steroid. Metabolit sekunder tersebut dapat berperan sebagai antibakteri, antikanker, antiinflamasi, dan antivirus. Tujuan penelitian ini adalah mengarakterisasi metabolit sekunder dan menguji aktivitas antibakteri fraksi etil asetat terhadap Staohylococcus aureus menggunakan metode difusi sumuran. Hasil interpretasi FTIR pada isolat menunjukkan adanya serapan gugus O-H melebar (3448,72 cm-1), =C-H (3016,67 cm-1), C-H alifatik (2927,94 cm-1), C-O alkohol (1118,71 cm-1); C=C olefin (1631,78 cm-1) C-X halogen (617,22- 410,84). Kehadiran gugus-gugus fungsi tersebut dan hasil uji fitokimia dapat diduga bahwa isolat merupakan senyawa olefin dari kelompok terpenoid. Hasil uji aktivitas antibakteri menunjukkan bahwa fraksi etil asetat menghambat bakteri Staphylococcus aureus pada konsentrasi 50 µg/µL dan 25 µg/µL masing-masing menunjukkan kapasitas kuat dalam menghambat bakteri, sedangkan pada konsentrasi 12,5 µg/µL terindikasi lemah; dan 6,25 µg/µL tidak menunjukkan aktivitas dalam menghambat bakteri S. aureus Hasil uji fitokimia fraksi etil asetat memberikan respon positif untuk senyawa terpenoid dan alkaloid.

Keywords


Spons; Haliclona; Antibakteri; Staphylococcus aureus

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DOI: https://doi.org/10.29103/jtku.v13i1.15596

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