Reducing Potato Cyst Nematode Attack on Granola L. with in-Vitro and ex-Vitro G0 Seed Production

Rindra Yusianto; Marimin Marimin

doi:10.29103/agrium.v21i2.16455

Reducing Potato Cyst Nematode Attack on Granola L. with in-Vitro and ex-Vitro G0 Seed Production

Rindra Yusianto, Marimin Marimin

Abstract

Potato Cyst Nematode/PCN (Globodera rostochiensis) attack is the most critical risk in potato farming. The attack was marked by a decrease in the production of up to 60%. The objectives of this study were to reduce postharvest losses due to PCN attacks on Granola L and to compare yields from G2 and G0 seeds. This research was conducted in 5 districts (N1, N2, N3, N4, and N5) in Wonosobo Regency, Central Java, Indonesia. The method used in this study was the comparison of yields from G2 and G0 seeds in the same area, considering temperature (V1) and altitude (V2). The results showed that Granola L from G2 seeds for 100 days after planting was 15.9 tons/ha, while the results for G0 seeds in the same field increased to 12.18 tons/ha. In G2 seeds, 52 cysts/100 ml of soil were obtained, while in G0 seeds, cysts were not accepted. Combining G0 seeds in vitro and ex-vitro on Granola L can reduce PCN attacks and increase yields by up to 73%. Conducting experiments with other cultivars, such as the Atlantic and Agria, is necessary for further research.

Keywords

Ex-vitro, Granola L, In-vitro, Potato cyst nematode.

Full Text:

PDF

References

Aires, A., Carvalho, R., Barbosa, M. dan C., & Rosa, E. (2009). Suppressing Potato Cyst Nematode, Globodera rostochiensis, with Extracts of Brassicacea Plants. American Journal Of Potato Research, 86(April 2015), 327–333. https://doi.org/10.1007/s12230-009-9086-y

Delate, K., & Dewitt, J. (2004). Building a farmer- centered land grant university organic agriculture program : A Midwestern partnership. Renewable Agriculture and Food Systems,

(2), 80–91. https://doi.org/10.1079/RAFS200365

Dutt, M., Dhekney, S. A., Soriano, L., Kandel, R., & Grosser, J. W. (2014). Temporal and spatial

control of gene expression in horticultural crops.

Horticulture Research, 1(7), 1–17. https://doi.org/10.1038/hortres.2014.47

Griffin, D., Holgado, R., & Ollivier, F. (2015).

Quarantine Nematodes in Potato : Practical Solutions Using Molecular Tools. Potato Research, 2014, 365–366.

https://doi.org/10.1007/s11540-014-9281-1

Hardjomidjojo, H., Yusianto, R., Marimin, M., & Suprihatin, S. (2022). Sustainable Agro-industry Logistics Solutions using Spatial Analysis. Operations and Supply Chain Management,

(1), 41–55. https://doi.org/10.31387/oscm0480329

Ibrahim, I. A., Emara, H. A., Nower, A. A., & Abodiab, A. Y. (2016). In vitro Cultivation of

Potato Plants. International Journal of Current

Microbiology and Applied Sciences, 5(12), 858–

Indarti, S., RTP, B., Mulyadi, & Triman, B. (2004).

First record of potato cyst nematode Globodera rostochiensis in Indonesia. Australasian Plant Pathology, 33(11), 325–326. https://doi.org/10.1071/AP04018

Janssen, G. J. W., Norel, A. Van, Verkerk-Bakker, B.,

& Janssen, R. (1995). Resistance to the root- knot nematodes Meloidogyne hapla and M . Chitwood in potato and wild. Potato Research,

(1), 353–362.

Lisnawita, L. (2017). Nematoda Aphelencoides besseyi : Status, Potensi Kerusakan, dan Strategi Pengendalian. Simposium Nasional Fitopatologi, 1, 1–16.

Mugniéry, D. (2008). The Canon of Potato Science :

Root-knot Nematodes. Potato Research,

(2007), 263–265. https://doi.org/10.1007/s11540-008-9048-7

Mulyadi, Rahayu, B., Triman, B., & Indarti, S. (2003).

Identifikasi Nematoa Sista Kuning (Globodera rostochinensis) pada Kentang di Batu, Jawa Timur. Jurnal Perlindungan Tanamanan Indonesia, 9(1), 46–53.

Muthulakshmi, M., Kumar, S., & Subramanian, S. (2012). Biology of entomopathogenic

nematodes Heterorhabditis sp . and Steinernema

spp. Journal of Biopesticides, 5(March 2012),

–61.

Nagachandrabose, S. (2020). Management of Potato Cyst Nematodes Using Liquid Bioformulations of Pseudomonas fluorescens, Purpureocillium

platinum, and Trichoderma viride. Potato

Research, 1(2), 1–18.

Pavlista, A. D. (2011). Growth Regulators Increased Yield of Atlantic Potato. American Journal Of Potato Research, 88(10), 479–484. https://doi.org/10.1007/s12230-011-9214-3

Sereno, P. C., & Danchin, E. G. J. (2014). Parasitic success without sex–the nematode experience.

J. Evol. Biol., 27(1), 1323–1333. https://doi.org/10.1111/jeb.12337

Supramana, S., Sinaga, M. S., Nurmansyah, A., & Mutaqin, K. H. (2019). The Morphology and Morphometry of Potato Cyst Nematodes (Globodera spp.) from Dataran Tinggi Dieng, Central Java. Jurnal Fitopatologi, 15(Maret

, 77–84. https://doi.org/10.14692/jfi.15.2.

Viaene, N. (2015). Nematodes : An Increasing Threat for the Potato Crop in Europe ? Potato Research, 1(2014), 335–338. https://doi.org/10.1007/s11540-015-9284-6

Wibowo, C., Haryanti, P., & Wicaksono, R. (2019).

Effect of Blanching Method and Soaking Solution on the Properties of Potato Flour Produced from Variety Granola. IOP Conf. Series: Earth and Environmental Science,

(1), 1–8. https://doi.org/10.1088/1755-

/255/1/012021

Yusianto, R., Shidiq, G. F., Suprijono, H., & Marimin. (2023). Adaptive Models for Determining Cropland Suitability and Total Harvest Prediction for Potato Using Iot. Potato Journal,

(1), 26–37.

DOI: https://doi.org/10.29103/agrium.v21i2.16455