Biochemical analysis of candidate probiotic bacteria was isolated from the digestive tract of the Banana shrimp (Penaeus merguiensis)

Hendra Raharja, Anis Zubaidah, Dony Prasetyo

Abstract


In nature, there are many types of bacteria that can act as probiotics. These bacteria can come from soil, water, plants or even the digestive tract of animals. This study aims to analyze the biochemical characteristics of probiotic candidate bacteria originating from the digestive tract of giant prawns. The method used is a descriptive explorative method to perform biochemical analysis of the types of probiotic bacteria derived from jerbung shrimp. The biochemical analysis included the Catalase test, Oxidase test, Urea test, Citrate test, TSIA, Methyl red, Voges-Proskaeurs, and sugar fermentation test (D-Glucose, Sucrose, Inositol, Manisitol. Laboratory analysis results showed that the isolated bacteria had morphological characteristics rod-shaped, gram-positive, does not produce catalase and oxidase enzymes, but can produce citric enzymes. These bacteria are acidic, capable of fermenting glucose, sucrose, inositol, and mannitol. The conclusion of this study, probiotic candidate bacteria from the test results showed compatibility with Lactobacillus sp bacteria.

Keywords: isolation; probiotics; shrimp


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Ako, S.E., Akum, E.A., Kenfon, C.N., Pokam, T.B., and Assob, J.C.N. 2020. Fecal gram strain morphotype and their distribution patterns in a Cameroonian cohort with and without HIV infection. Scientific African, 8, e00376.

Ayyas, M.M., Abdalla, A.K., Alkalbani, N.S., Baig, M.A., Turner, M.S., Liu, S.Q., and Shah, N.P. 2021. Invited review: Characterizationof new probiotics from dairy and nondairy products-Insights inti acid tolerance, bile metabolism and tolerance, and adhesion capability. Journal of Dairy Science

Colantuono, A., D’Inceccco, P., Fortina, M.G., Rosi, V., Ricci, G., dan Pallegrino, L. 2020. Milk substrates influence proteolytic activity of Pseudomonas fluorescens strains. Food Control, 111, 107063

Holt, J.G., Krieg, N.R., Sneath, P.H.A., Stanley, J.T. and William, S.T. 1994. Bergey’s Manual of Determinative Bacteriology. Williams and Wilikins, Baltimore, 786-788

Ilham, BI., Darmayasa, IBG., Kawuri, R. 2014. Isolasi dan identifikasi bakteri pelarut fosfat potensial pada tanah konvensional dan tanah organik. Jurnal Simbiosis, 2(1):173-183

Janković, T., Frece J., Abram M., and Gobin I. 2012. Ag-gregation ability of potential probiotic Lactobacillus plantarum. Int J. Sanitary Eng Res 6:19-24.

Kim, S., Jeon, H., Han, H., and Hur, J.W. 2021. Evaluation Bacillus albus SMG-1 and B. safensis SMG-2 were isolated from Saemangeum as probiotic for whiteshrimp culture (Litopenaeus vanname). Aquaculture Report, 20 ,100743

Meyers, A., Furtmann, C., and Jose, J. 2018. Direct optical density determination of bacterial cultures in microplates for high-throughput screening applications. Enzyme and Microbial Technology, 118 (July), 1–5.

Mulianto, Diki., Widyowati., Raharja, Hendra., Zubaidah, Anis. 2021. Aktivitas Proteolitik Bakteri Kandidat Probiotik Dari Saluran Pencernaan Udang Jerbung, Panaeus merguiensis. Jurnal Riset Akuakultur, 16(04), 221-229.

Mustaqim., Reza, M, R., Bernadeta. 2014. Isolasi dan Karakterisasi Bakteri Probiotik Pada Saluran Pencernaan Ikan Lais. JOM FMIPA, Vol. 1, No. 2.

Moussaid, F., Barnossi, A. E., Chahmi, N., and Housseini, A.I. 2019. Screening and selection of new microbial anti-Candida. Materials Today: Proceedings, 13, 1049-1054.

Nopitawati, T. 2010. Seleksi bakteri probiotik dari saluran pencernaan untuk meningkatkan kinerja pertumbuhan udang vaname (Litopenaeus vannamei). Tesis. IPB

Pahlawi, I.M.H., and Satyantini, W. H. 2019. Pathogenicity test of Pseudomonas sp. in white shrimp (Litopanaeus vannamei) as a probioitic candidate. Journal of Aquaculture and Fish Health, 8(2), 92-98.

Prihanto, A.A., Laksono, H.D., Jaziri, A.A., Nurdiani, R., Pradarameswari, K.U. 2018. Isolasi Dan Identifikasi Bakteri Endofit Mangrove Sonneratia alba Penghasil enzim Gelatinase Dari Pantai Sendang Biru, Malang, Jawa Timur. Indonesian Journal of Halal. Universitas Brawijaya.

Sousa, A.M., Pereira, M.O., and Lourenço, A. 2015. MorphoCol: An ontology-based knowledgebase for the characterisation of clinically significant bacterial colony morphologies. Journal of Biomedical Informatics, 55, 55–63.

Suo, X., Huang, S., Wang, J., Fu, N., Jeantet, R., and Chen, X.D. 2021. Effect of culturing lactic acid bacteria with varying skim milk concentration on bacteria survival during heat treatment. Journal of Food Engineering, 294, 110396.

Sutrisna, R. 2013. Karakteristik Isolat Bakteri Asam Laktat dari Usus Itik Terhadap Escherichia coli dan Salmonella pullorum. Makalah. Disajikan dalam Seminar Nasional Sains dan Teknologi V oleh Lembaga Penelitian Universitas Lampung.

Suwito, Widodo. 2010. Bakteri yang Sering Mencemari Susu: Deteksi, Patogenesis, Epidemologi, dan Cara Pengendaliaannya. Jurnal Litbang Pertanian, 29 (3), 96-100.

Trimudita, F, R., Djaenudin. 2021. Enkapsulasi Probiotik Lactobacillus sp. Menggunakan Dua Tahap Proses. Serambi Engineering, Vol. IV, No.2, Hal. 1832-1841.

Ulfa, A., Suarsini, E., al Muhshar, M. H. I. 2016. Isolation and Mercury Sensitivity Test of Bacterias Isolated from Waste Disposal in Gold Mining Area in West Sekotong of West Lombok Region: Preliminary Study. Proceeding Biology Education Conference 13(1) : 793-799.




DOI: https://doi.org/10.29103/aa.v10i2.9062

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