Identification of bioactive compounds in extract fraction red seaweed (Eucheuma cottonii)
Abstract
Seaweed is a biological resource that is rich in nutrients and bioactive compounds. This study aims to determine qualitatively and quantitatively the bioactive compounds (flavonoids, alkaloids, steroids/terpenoids, saponins and phenols) from extracts and fractions of Eucheuma cottonii. The method used is an experiment by conducting a series of experiments by extracting and fractionating E. cottonii and the data were analyzed descriptively. The treatments used were organic solvents with different polarity levels (methanol, n-hexane, ethyl acetate, and butanol) for extraction and fractionation. Parameter analysis consisted of qualitative phytochemical tests (flavonoids, alkaloids, steroids/terpenoids, saponins and phenols) and quantitatively determined the total content of alkaloids, flavonoids, and saponins. The results of the phytochemical test of methanol extract qualitatively produced alkaloids, flavonoids, steroids/terpenoids, and saponins. The n-hexane fraction contains steroids/terpenoids and saponins. The ethyl acetate fraction contains alkaloids, flavonoids and steroids/terpenoids and the butanol fraction contains alkaloids. The results of quantitative analysis of the n-hexane extract fraction showed that the content of saponins was 0.23%, alkaloids and flavonoids were 0.16% and 0.09% in the ethyl acetate fraction, while the alkaloid content was 0.09% in the butanol fraction.
Keywords: fractionation; organic solvent; phytocemical; quantitative
Full Text:
PDFReferences
[KKP] Kementerian Kelautan dan Perikanan. 2018. Produktivitas Perikanan Indonesia. Jakarta (ID): Kementerian Kelautan dan Perikanan.
Alindra P., Lena J. Damongilala., Hanny W. Mewengkang. 2018. Kandungan Antioksidan Pada Rumput Laut Eucheuma Spinosum Yang Diekstrak Dengan Metanol Dan Etanol. Jurnal Media Teknologi Hasil Perikanan. 6(1): 197-201.
Arsa, Made. 2016. Proses Pencoklatan (Browning Process) Pada Bahan Pangan. Denpasar: Universitas Udayana.
Brown EM, Allsopp PJ, Magee PJ, Gill CI, Nitecki S, Strain CR, Mcsorley EM. 2014. Seaweed and human health. Nutrition Reviews. 72(3) : 215-216.
Harborne JB. 1996. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Terbitan Kedua. Bandung: ITB.
Lantah, P. L. Montololu L. A. D. Y, dan Reo R. A. 2017. Kandungan Fitokimia Dan Aktivitas Antioksidan Ekstrak Metanol Rumput Laut Kappaphycus Alvarezii. Jurnal Media Teknologi Hasil Perikanan. 5(3): 167-173.
Lenny, S. 2006. Senyawa Flavonoida, Fenil Propanoida dan Alkaloida, Karya Ilmiah, FMIPA. Medan: USU.
Mierziak, J., Kostyn, K., Kulma, A. 2014. Flavonoids as important molecules of plant interactions with the environment. Molecules. 19(10): 16240-16265.
Nwaoguikpe, R.N., Braide, W., & Ezejiofor, T.I.N. 2010. The effect of Aloe vera plant (Aloe barbadensis) extracts on sickle cell blood. African Journal of Food Science and Technology. 1(3): 58-63.
Pangestuti, Indria, E. Sumardianto, Ulfah. A. 2017. Skrining Senyawa Fitokimia Rumput Laut Sargassum sp. dan Aktivitasnya Sebagai Antibakteri Terhadap Staphylococcus aureus dan Eschericia coli. Jurnal Fisheries Science and Technology. 12(2): 98-102.
Purba, R.D. 2001. Analisis Komposisi Alkaloid Daun Handeuleum (Graptophyllum pictum) yang Dibudidayakan dengan Taraf Nitrogen yang Berbeda [Skripsi]. Bogor: Institut Pertanian Bogor.
Robinson, T. 1995. Kandungan Organik Tumbuhan Tinggi. Bandung: ITB.
Saidi, nurdin. Binawati ginting. Murniana. Mustanir. 2018. Analisis Metabolit Sekunder. Aceh: Syiah Kuala university.
Sangi, M., M.R.J. Runtuwene., H.E.I. Simbala., V.M.A. Makang. 2008. Analisis Fitokimia Tumbuhan Obat di kabupaten Minahasa Utara. Chem. Prog, 1(1): 47-53.
Setyowati W.A.E. Ariani S.R.D. Ashadi, Mulyani B. and Rahmawati C.P. 2014. Skrining Fitokimia Dan Identifikasi Komponen Utama Ekstrak Metanol Kulit Durian (Durio zibethinus Murr.) Varietas Petruk. Seminar Nasional Kimia dan Pendidikan Kimia VI: 271-280.
Simaremare, E. S. 2014. Skrining Fitokimia Ekstrak Etanol Daun Gatal (Laportea decumana (Roxb) Wedd). Pharmacy.11(1): 98-107.
Sjahid LR. 2008. Isolasi dan Identifikasi Flavonoid Dari Daun Dewandaru (Eugenia uniflora L). [Skripsi]. Surakarta: Universitas Muhammadiyah.
Titis, M. B. M., E. Fachriyah, dan D. Kusrini. 2013. Isolasi, Identifikasi dan Uji Aktifitas Senyawa Alkaloid Daun Binahong (Anredera cordifolia (Tenore) Steenis). Chem. Info. 1 (1):196-201.
Vincken, J.P., L. Heng, A. De Groot, & J.H. Gruppen. 2017. Saponins, classification and occurrence in the plant kingdom. Phytochem. 68(3): 275-297.
Yanuarti R, Nurjanah, Anwar E, Hidayat T. 2017. Profil fenolik dan aktivitas antioksidan dari ekstrak rumput laut Turbinaria conoides dan Eucheuma cottonii. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(2): 230-237.
DOI: https://doi.org/10.29103/aa.v9i2.6164
Article Metrics
Abstract Views : 254 times
PDF Downloaded : 223 times
Refbacks
- There are currently no refbacks.
Copyright (c) Acta Aquatica: Aquatic Sciences Journal
This work is licensed under a Creative Commons Attribution 4.0 International License.