Using papain enzyme to improve feed protein utilization for kissing gourami (Helostoma temminckii)
Abstract
The aim of this research was to determine of optimum dosage of papain enzyme in feed for kissing gourami. This research used Completely Randomized Design non-factorial, consist of four treatments and three replications, namely: P0 (wihout papain supplementation in feed/as a control), P1 (1.5% papain in feed), P2 (3.0% papain in feed), and P3 (4.5% papain in feed). Kissing gourami with initial body weight of 0.51+0.07 g were stocked in each aquarium (volume 20 L of water) of 2 fish per litre of water. Fish reared for 45 days. During rearing time, fish were fed three times a day at satiation. Observed variable were growth, feed efficiency, protein retention, survival rate, and water quality of rearing media of kissing gourami. The growth, feed efficinecy, protein retention, and survival of kissing gourami were analyzed by analysis of variance. If the data significant different were analyzed by Least Significant Different test. Water quality of rearing media were descriptive analysis. The result showed that addition of 1.5% papain enzyme in feed result in the growth, feed efficiency, protein retention, and survival of kissing gourami were highest. The weight growth of fish of 0.44 g per fish, feed efficiency of 38.66%, protein retention of 23.60%, and survival of 97.78%. Water quality of rearing media were still in tolerance range for kissing gourami.
Keywords: Feed efficiency; Growth; Kissing gourami; Papain enzyme; Protein retention
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Ahmad, N. 2016. Analisa pemberian dosis pakan yang berbeda terhadap pertumbuhan ikan tambakan (Helostoma temminckii). Jurnal Agroqwa. 14 (2): 77-80.
Amalia, R., Subandiyono, A., dan Arinis. 2013. Pengaruh penggunaan papain terhadap tingkat pemanfaatan protein pakan dan pertumbuhan lele dumbo (Clarias gariepinus). Journal of Aquaculture Management and Technology. 2(1): 136-143.
Ananda, T., Rachmawati, D., dan Samidjan, I. 2015. Pengaruh papain pada pakan buatan terhadap pertumbuhan ikan patin (Pangasius hypopthalmus). Journal of Aquaculture Management and Technology. 4 (1): 47-53.
Arifin, O.Z., V.A., Prakoso, B., dan Pantjara. 2017. Ketahanan ikan tambakan (Helostoma temminkii) terhadap beberapa parameter kualitas air dalam lingkungan budidaya. Jurnal Riset Akuakultur. 12 (3): 241-251.
Dongoran, D.S. 2004. Pengaruh aktivator sistein dan natrium klorida terhadap aktivitas papain. Jurnal Sains Kimia. 8 (1): 30-35.
Fadli, J., Sunaryo, A., dan Djunaedi. 2013. Pemberian enzim papain pada pakan komersil terhadap pertumbuhan dan efisiensi pakan ikan kerapu macan (Epinephelus fuscoguttatus). Journal of Marine Research. 2(3): 50-57.
Hopkins, K.D. 1992. Reporting fish growth: A review of the basics. Journal of the World Aquaculture Society. 23(3): 173-179.
Hutabarat, J., Rachmawati, D., dan Samidjan, I. 2016. Pengaruh enzim protease papain dalam pakan buatan terhadap pertumbuhan dan net protein utilization benih lele sangkuriang yang dibudidaya di Desa Wonosari, Kecamatan Bonang, Kabupaten Demak. PENA Akuatika. 14 (1): 25-35.
Irawati, D., Rachmawati, D., dan Pinandoyo. 2015. Performa pertumbuhan benih ikan nila hitam (Oreochromis niloticus Bleeker) melalui penambahan enzim papain dalam pakan buatan. Journal of Aquaculture Management and Technology. 4 (1): 1-9.
Irawati, R. 2016. Karakterisasi pH, suhu, dan konsentrasi substrat pada enzim selulase kasar yang diproduksi oleh Bacillus circulans. Skripsi. Univesitas Islam Negeri Maulana Malik Ibrahim Malang.
Mareta, R.E., Subandiyono, dan Hastuti, S. 2017. Pengaruh enzim papain dan probiotik dalam pakan terhadap tingkat efisiensi pemanfaatan pakan dan pertumbuhan ikan gurami (Osphronemus gouramy). Jurnal Sains Akuakultur Tropis. 1(1): 21-30.
Nasution, E.P.A., Muchlisin, Z.A., dan Yulvizar, C. 2017. Pengaruh kombinasi enzim papain dan bromelin terhadap pemanfaatan pakan dan pertumbuhan ikan nilem Osteochillus vittatus. Jurnal Ilmiah Mahasiswa Kelautan dan Perikanan Unsyiah. 2(3): 339-348.
Raharjo, E.I., Rachimi., dan Riduan, A. 2016. Pengaruh padat tebar yang berbeda terhadap pertumbuhan dan kelangsungan hidup benih ikan biawan (Helostoma temminckii). Jurnal Ruaya. 4 (1): 45-53.
Susanti, R., dan Fibriana, F. 2017. Teknologi Enzim. Yogyakarta: CV. AndiOffset.
Watanabe, T. 1988. Fish Nutrition and Mariculture. JICA Textbook The General Aquaculture Course. Departmen of Aquatic Biosciences Tokyo University of Fisheries.
Zonneveld, N., Huisman, E.A., dan Boon, J.H. 1991. Prinsip-Prinsip Budidaya Ikan. PT. Gramedia Pustaka Utama, Jakarta.
DOI: https://doi.org/10.29103/aa.v9i1.5885
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