Characterisation and antibiotic susceptibility pattern of bacterial isolates obtained from some shellfish sold in Lagos-West, Nigeria

C. E. Oramadike, O. O. Olajuyigbe, Esak Esther Amusan, R. O. Esangbedo, M. O. Awoyale

Abstract


Shellfish are rich in essential nutrients and are widely accepted globally. The bacterial contamination, antibiotic susceptibility and heavy metal concentrations on some shellfish muscles above the tolerance permissible limit by WHO/FAO require an in-depth study. An investigative study was carried out on tiger shrimp (Penaeus monodon), pink shrimp (Penaeus notialis) and Lagoon crabs (Callinectes amnicola) obtained from Makoko fish market/landing site on their bacterial quality, antibiotic susceptibility patterns and heavy metal accumulation of bacteria isolated were carried out using standard methods. The highest Total bacterial count (2.48±0.02107cfu/g) was observed in Lagoon crab (C. amnicola) while the lowest count (1.20±0.02107cfu/g) was observed in tiger shrimp (P. monodon). However, the highest Total Faecal count (1.42±0.02104cfu/g) was observed in pink shrimp (P. notialis) while the lowest count was observ ed in P. monodon (1.13±0.03104cfu/g). The bacterial isolates were molecularly identified as (Morganella morganii and Proteus vulgaris) isolated from tiger shrimp, Lagoon crabs had (Proteus mirabilis) while pink shrimp showed (Alcaligenes faecalis). The isolates were 100% susceptible to ciprofloxacin, azithromycin and erythromycin and resistant to cefotaxime, cefuroxime, imipenem/clastatin, augmentin and nitrofurantoin. The mean heavy metals concentration was as follows zinc>iron>copper>nickel>chromium>manganese > Cadmium while Lead and cobalt were not detected in the samples. The study has shown a possible unhygienic environment indicative of the bacteria isolated, a possible environmental spread of antibiotic-resistant bacteria and a heavy metal-contaminated water body. There is a serious need for constant monitoring to lessen future health problems for humans in and around the environment.

Keywords: antibiotic-resistant; contamination; Makoko


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References


Adams, S.M. 2002. Biological indicators of aquatic ecosystem stress: Introduction and overview. In Biological Indicators of Aquatic Ecosystem Stress. American Fish Society Bethesda Maryland 1-11.

Adeyemi, A., Enyinnia, V., Nwanze, R., Smith, S., and Omonigbehin, E. 2008. Antimicrobial susceptibility of potentially pathogenic halophilic Vibrio species isolated from seafoods in Lagos, Nigeria. African Journal of Biotechnology, 7: 3791-3794.

Afolayan, O.A., Moruf, R.O., and Lawal-Are, A.O. 2020. Bacterial Contamination and Heavy Metal Residues in Frozen Shellfish Retailed Within Lagos Metropolis, Nigeria. Science World Journal, 15: 1597-6343.

AOAC (Association of Official Analytical Chemists). 1980. Official methods of analysis. 534. W. Horwitz (Ed.). Arlington, V.A, Washington DC: AOAC.

Bojarczuk, A., Jelonkiewicz, L., and Lenart-Boron A. 2018. The effect of anthropogenic and natural factors on the prevalence of physicochemical parameters of water and bacterial water quality indicators along the river Bialka, Southern Poland. Environmental Science and Pollution Research International, 25: 10102-10114.

Bruins, M.R., Kapal, S., and Oehme, W.F. 2000. Microbial resistance to metals in the environment. Ecotoxicology and Environmental Safety, 45: 198-207.

Cheesbrough, M. 2002. District laboratory practices in tropical countries, Part II. United Kingdom: Cambridge University Press, 182-187.

CLSI (Clinical and Laboratory Standards Institute). 2018. Performance Standards for Antimicrobial Susceptibility testing. 28th ed. CLSI supplement M100. Wayne, PA: CLSI; p15.

Conlan, S., Kong, H.H., and Segre, J.A. 2012. Species-Level Analysis of DNA Sequence Data from the NIH Human Microbiome Project, 7:10.

Ekwealor, P.A., Ugwu, M.C., Ezeobi, I., Amalukwe, G., Ugwu, B.C., Okezie, U., Stanley. C., and Esimone, C. 2016. Antimicrobial evaluation of bacterial isolates from urine specimens of patients with complaints of urinary tract infections in Awka, Nigeria. International Journal Microbiology, Article ID 9740273, 6.

Elbashir, S., Parveen, P., Schwarz, J., Rippen, T., Jahncke, M., and Depaola, A. 2018. Seafood pathogens and information on antimicrobial resistance: A review. Food Microbiology, 70: 85-93.

Falomir, M.P., Gozalbo, D., and Rico, H. 2010. Coliform bacteria in fresh vegetables: From cultivated lands to consumers. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, 2: 1175–1181.

Farkas, A., Salanki, J., and Specziar, A. 2002. Relation between growth and the heavy metal concentration in organs of Bream Abramis brama L. populating Lake Balaton. Archives Environmental Contamination Toxicology, 43: 236-243.

Hembach, N., Schmid, F., Alexander, J., Hiller, C., Eike, T.R., and Schwart, J. 2017. Occurrence of the mcr-1 colistin resistance gene and other clinically relevant antibiotic resistance genes in microbial populations at different municipal wastewater treatment plants in Germany. Frontiers in Microbiology, 8: 1282.

ICMSF (International Commission on Microbiological Specifications for Foods). 1986. Sampling plans for fish, shellfish: Microorganisms in foods 2. Sampling for microbiological analysis: principles and specific Applications 2nd Edn. University of Toronto Press, Toronto, Canada. p 181-195.

Jumbo, A., Wegwu, M.O., Belonwu, D.C., and Okerenta, B.M. 2015. Assessment of heavy metal concentrations of selected fin and shell fish from Ogoniland. Journal of Environment and Earth Science, 5: 15-19.

Kumar, S., Stecher, G., Li. M., Knya, Z.C., and Tamura, K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution, 2018; 35: 1547-1549.

Lima de Silva, A.A., de Carvalho, M.A., de Souza, S.A. Dias, P.M., Filho, R.G., Saramago, C.S., Bento, C.A., and Hofer, E. 2012. Heavy metal tolerance (Cr, Ag AND Hg) in bacteria isolated from sewage. Brazilian Journal of Microbiology, 43: 1620-31.

Liu, H., Zhu, J., Hu, Q., and Rao, X. 2016. Morganella morganii, a non-negligent opportunistic pathogen (Review). International Journal of Infectious Diseases, 50: 10-17.

Mohamed, M.A., Al Shabeeb, S., Al Ramadhan, G.H., and Imran, P.M. 2017. Isolation of enterobacteriaceae from raw seafoods sold in fish markets in eastern province of Saudi Arabia. International Journal of Advanced Research, 5: 1711-1718.

Omoya, F.O., and Ajayi, A.T. 2020. Assessment of the microbial quality of seafood and effects of salt concentration and temperature on isolated microorganisms. Journal of Microbiology and Antimicrobials, 12: 17-31.

Pérez-Etayo, L., González, D., Leiva, J., and Vitas, A.I. 2020. Multidrug-Resistant Bacteria Isolated from different Aquatic Environments in the North of Spain and South of France. Microorganisms, 8: 1425.

Stecher, G., Tamura, K., and Kumar, S. 2020. Molecular Evolutionary Genetics Analysis (MEGA) for macOS. Molecular Biology and Evolution, 37: 1237–1239.

Tosun, S.Y., Ucok D., and Mol, A.S. 2016. Isolation of Salmonella spp and other members of Enterobacteriaceae from horse Mackerel (Trachurus trachurus), sold in public markets of Istanbul, Turkey. Journal of Food and Health Science, 2: 82-89.

Tula, M.Y., Enabulele, O.I., and Ophori, E.A. 2021. Prevalence of parasite and MDR Enteropathogenic bacteria from water sources in Adamawa- North senatorial zone, Adamawa State Nigeria. Unpublished From GenBank, NCBI.

Udoekong, N.S., Bassey, B.E., Asuquo, A.E., Akan, O.D., and Ifeanyi, C.I.C. 2021. Multi-Drug Resistance Genes associated with some Gram-Negative Bacteria Isolates from Shellfish in Iko and Douglas River Estuaries, in Nigeria. European Journal of Biology, 2: 19-27.

USDA/FSIS (United States Department of Agriculture/ Food Safety and Inspection Service). 1998. Microbiology Laboratory Guidebook, 3rd Ed., Washington, DC: USDA–FSIS.

Watts, J.E.M., Schreier, H.J., Lanska, L., and Hale, M.S. 2017. The Rising Tide of Antimicrobial Resistance in Aquaculture: Sources, Sinks and Solutions. Marine Drugs, 15:158.

Wicksten, M., De Grave, S., France, S., and Kelley, C. 2017. Presumed filter-feeding in a deep-sea benthic shrimp (Decapoda, Caridea, Stylodactylidae), with records of the deepest occurrence of carideans. ZooKeys, 646: 17-23.




DOI: https://doi.org/10.29103/aa.v11i3.15878

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