The effectiveness of activated charcoal in feed on histopathological features of the spleen of tilapia (Oreochromis niloticus) exposed to organophosphate insecticides

Nurhayati Nurhayati, Shahibul Auzan, Lia Handayani, Azwar Thaib, T M Haja Almuqaramah, Faisal Syahputra

Abstract


Excessive and periodic use of insecticides has a negative impact on aquatic areas and the biota in them. Pesticides that accumulate in the aquatic environment affect the life non target organisms such as fish. In addition, pesticides can cause damage to the gills, liver, intestines and spleen. This organ plays an important role in the immune system. Thus, an alternative is needed to overcome this problem, one of which is the addition of activated charcoal in the feed. The aims of study to determine the effect of the use of activated charcoal in feed on the histopathological description of the spleen of tilapia exposed to organophosphate pesticides. The study was conducted experimentally by applying 4 treatments and 2 replications as the treatments used included T1 (0% activated charcoal); T2 (1% activated charcoal); T3 (2% activated charcoal); T4 (3% activated charcoal). The test fish used were tilapia measuring 7 ± 0,4 cm long with a stocking density of 30 fish/container. Parameters observed were histopathological description of tilapia spleen at pre exposure, post exposure and after administration of activated charcoal. The results of observations of tissue damage were analyzed descriptively qualitatively and presented in the form of images. The results showed that the addition of 2% activated charcoal (T3) in the feed was able to reduce damage to the spleen due to exposure to organophosphate insecticides.

Keywords: Activated charcoal; feed; insecticide; spleen


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Adharini, R. I., Suharno, D., & Hartiko, H. (2016). Pengaruh Kontaminasi Insektisida Profenofos terhadap Fisiologis Ikan Nila Merah (Oreochromis sp.). Jurnal Manusia Dan Lingkungan, 22(2), 365–373.

Alfis, N. F., Handayani, L., & Nurhayati. (2022). Gambaran Histologi Insang Ikan Nila (Oreochromis niloticus) Yang Terpapar Pestisida Golongan Organofosfat. Jurnal Tilapia, 3(1), 28–37.

Banaee, M. (2013). Physiological Dysfunction in Fish After Insecticides Exposure (Stanislav Trdan (ed.)). https://www.intechopen.com/books/3055

Banaee, M., Davoodi, M. H., & Zoheiri, F. (2013). Histopathological Changes Induced by Paraquat on Some Tissues of Gourami Fish (Trichogaster trichopterus). Open Veterinary Journal, 3(1), 36–42. http://www.ncbi.nlm.nih.gov/pubmed/26623309%0Ahttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC4629585

Darwis, V., Muslim, C., & Anugerah, I. S. (2021). Perilaku Petani Dalam Penggunaan Pestisida Pada Budidaya Bawang Merah di Kabupaten Cirebon. Jurnal of Food System and Agribusines, 5(2), 166–176.

He, Y., Wang, E., Wang, K., Wang, J., Fan, W., Chen, D., & Yang, Q. (2021). Morphology of the Spleen in Oreochromis niloticus : Splenic Subregions and the Blood-Spleen Barrier. Jurnal Animal, 1–12.

Kapila, V., Wehrle, C. J., & Tuma, F. (2022). Physiology, Spleen. StatPearls Publishing LLC. https://www.ncbi.nlm.nih.gov/books/NBK537307/

Khairati, R., & Syahni, R. (2016). Respons Permintaan Pangan terhadap Pertambahan Penduduk di Sumatera Barat. Jurnal Pembangunan Nagari, 1(2), 19–36.

Melia, Y., Handayani, L., & Nurhayati. (2022). Gambaran Histologi Hati Ikan Nila (Oreochromis niloticus) Yang Terpapar Pestisida Golongan Organofosfat. Jurnal Tilapia, 3(1), 38–46.

Mubarokah, L., Tjahjaningsih, W., & Sulmartiwi, L. (2019). Efek Immunotoksik Logam Berat Merkuri Klorida (HgCl2) terhadap Perubahan Ukuran Melano - Makrofag Ginjal Ikan Mas (Cyprinus carpio). Journal of Aquaculture and Fish Health, 5(3), 126. https://doi.org/10.20473/jafh.v5i3.11334

Nugroho, B. Y. H., Wulandari, S. Y., & Ridlo, A. (2015). Analisis Residu Pestisida Organofosfat di Perairan Mlonggo Kabupaten Jepara. Jurnal Oseanografi, 4(3), 1–6.

Nurhayati, Mukarramah, T. M. H. Al, Defsuar, E., Handayani, L., & Muhazzir, S. (2022). Pemberian Pakan Bersuplemen Arang Aktif dari Tulang Ikan terhadap Reduksi Insektisida Diazinon dalam Tubuh Ikan Nila: Studi Kasus Histologi Insang Ikan Nila (Oreochromis niloticus). Jurnal Tilapia, 3(2), 29–34.

Nurhayati, Nazlia, S. N., Fattah, A. F., Pradinata, Y., Handayani, L., & Harun. (2021). Kinerja Pertumbuhan Ikan Gurami, Osphronemus goramy dengan Penambahan Arang Aktif Tulang Ikan Kambing-kambing dalam Pakan. Jurnal Media Akuakultur, 16(2), 87–93. https://doi.org/10.15578/ma.16.2.2021.87-93

Risna, F., Handayani, L., & Nurhayati. (2020). Pengaruh Penambahan Arang Aktif Tulang Ikan dalam Pakan terhadap Histologi Usus Ikan Nila (Oreochromis niloticus). Jurnal Tilapia, 1(2), 28–33.

Soares, P. R. L., Andrade, A. L. C. de, Santos, T. P., Silva, S. C. B. L. da, Souza, J. F. da S., Santos, A. R. dos, Souza, E. H. L. da S., Cunha, F. M. da, Teixeira, V. W., Cadena, M. R. S., Sa, F. B. de, Junior, L. B. de C., & Cadena, P. G. (2016). Acute and Chronic Toxicity of the Benzoylurea Pesticide, Lufenuron, in the Fish, Colossoma macropomum. Jurnal Chemosrphere, 161, 412–421. https://doi.org/10.1016/j.chemosphere.2016.07.033

Suharno. (2018). Revitalisasi Agrikultur Untuk Pertumbuhan Ekonomi: Perspektif Ekonomi Islam. Jurnal Ekonomi Islma, 141–155.

Thaib, A., Handayani, L., Hanum, A., Nurhayati, N., & Syahputra, F. (2021). Evaluating the addition of starry triggerfish (Abalistes stellaris ) bone charcoal as a feed supplement to the growth performance and intestinal villi length of nile tilapia (Oreochromis niloticus ). Jurnal Ilmu-Ilmu Perairan, Pesisir Dan Perikanan, 10(March), 194–200. https://doi.org/10.13170/depik.10.2.20367

Thaib, A., Reta, A., & Nurhayati. (2021). Pengaruh Pemberian Charcoal Tulang Ikan Kambing-Kambing (Abalistes stellaris) Pada Pakan Untuk Mereduksi Kadar Amonia Pada Media Budidaya Ikan Nila (Oreochromis niloticus). Jurnal T, 2(2), 73–80.

Wang, X., Xing, H., Jiang, Y., Wu, H., Sun, G., Xu, Q., & Xu, S. (2013). Accumulation, Histopathological Effects and Response of Biochemical Markers in The Spleens and Head Kidneys of Common carp Exposed to Atrazine and Chlorpyrifos. Jurnal Food and Chemical Toxicology, 62, 148–158. https://doi.org/10.1016/j.fct.2013.08.044

Wynn, T. A., Chawla, A., & Jeffrey W. Pollar. (2013). Macrophage biology in development, homeostasis and disease. Nature, 496, 445–455.

Zulfahmi, I., Muliari, & Mawaddah, I. (2017). Toksisitas Limbah Cair Kelapa Sawit terhadap Ikan Nila (Oreocrhomis niloticus Linneus 1758) dan Ikan Bandeng (Chanos-chanos Froskall 1755). Jurnal Agricola, 7(1), 44–55.

Zulfikar. (2017). Tingkat Penggunaan Pestisida pada Tanaman Bawang Merah di Kecamatan Anggeraja, Kabupaten Enrekang.




DOI: https://doi.org/10.29103/aa.v1i2.9223

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