Use of Durian leaf infused (Durio zibethinus) as natural anesthetic for Gouramy fish (Osphronemus gouramy) in dry transportation

Hany Handajani, Dony Prasetyo, Sri Dwi Hastuti, Muhammad Fahimuridlo


Purpose the research to know influence giving leaf durian infused
to anesthesia process gurami fish with weight 100-150 gr use
predetermined dosage by upper threshold test and lower
threshold test. Then, use formula EC-100 (Effektivity
Consentrastion) to know fainting time fish and conscious time
fish. On threshold test use among others concentrate 1000ppm,
2500ppm, 5000ppm and 7500ppm. That at concentration
7500ppm is upper threshold because gurami fish die by soaking
leaf Durian infused for 24 hours while at concentration 5000pm
is lower threshold because gurami fish not die by soaking leaf
Durian infused for 48 hours. Next to search concentration row
with use formula EC-100 and than available concentrate
5400ppm, 5900ppm, 6400ppm, 6900ppm and 7400ppm. The
concentration be used to research treatment, P1:5400ppm,
P2:5900ppm, P3:6400ppm, P4:6900ppm and P5:7400ppm. The
Method of this research is a complete random design (RAL) use
five treatments and three replications. The main parameter
observed lamely fainting time fish and conscious time fish. The
result fainting time fish (minute) on P1: 200, 67 P2: 198,33 P3:
196 P4: 193 and P5: 186,67 while results conscious time fish
(minute) on P1: 22 P2: 22,67 P3: 23,33 P4: 28,33 and P5: 30,33.
Next statistic test use method ANOVA and BNT test with level 5%
indicate significantly different, and 1 % indicate very differently
real. The obtainable conclusion that research use leaf durian
infusum to anesthesia process gurami fish very differently real to
fainting time and conscious time. The best treatment on P5
(7400ppm) because gurami fish fast to fainting time and long a
for conscious time.

Keywords: Anesthesia; Durian Leaf; Gouramy; Infused; Saponin

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